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Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds
Published on: January 7, 2019
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14-3-3ε protein-immobilized PCL-HA electrospun scaffolds with enhanced osteogenicity
G Rivero1, A A Aldana1,2, Y R Frontini Lopez3
1Instituto de Investigaciones en Ciencia y Tecnología de Materiales, INTEMA (UNMdP-CONICET), Mar del Plata, Argentina.
Journal of Materials Science. Materials in Medicine
|August 29, 2019
Summary
Nanofibrous biomimetic scaffolds incorporating 14-3-3 protein epsilon significantly enhance early osteogenic differentiation of human adipose-derived stem cells (hASCs). This protein-enhanced matrix shows promise for bone tissue regeneration applications.
Area of Science:
- Biomaterials Science
- Stem Cell Biology
- Tissue Engineering
Background:
- Adipose-derived stem cells (ASCs) are crucial for bone tissue regeneration due to their osteogenic potential.
- Biomimetic scaffolds can guide stem cell differentiation for improved bone formation.
- The role of specific proteins in enhancing early osteogenic differentiation on composite scaffolds requires further investigation.
Purpose of the Study:
- To investigate the effect of nanofibrous biomimetic matrices containing poly(ε-caprolactone) (PCL), nanometric hydroxyapatite (nHA), and 14-3-3 protein epsilon on human ASCs (hASCs) osteogenic differentiation.
- To evaluate the impact of the protein-enriched scaffold on early osteogenic markers.
Main Methods:
- Human ASCs were isolated and characterized using flow cytometry.
- Cells were induced to differentiate along adipogenic and osteogenic lineages.
- Nanofibrous scaffolds (PCL-nHA/protein) were fabricated and used for cell culture.
- Alkaline phosphatase (ALP) activity was measured as an early osteogenic marker.
Main Results:
- Cell adhesion and viability were similar across all tested scaffolds (PCL-nHA/protein, PCL-nHA, and neat PCL).
- The PCL-nHA/protein scaffold exhibited a fourfold higher ALP activity compared to the PCL-nHA scaffold.
- ALP activity was over five times higher in the PCL-nHA/protein scaffold compared to the neat PCL scaffold.
Conclusions:
- The incorporation of 14-3-3 protein epsilon into PCL-nHA nanofibrous scaffolds significantly promotes the early osteogenic differentiation of hASCs.
- These protein-functionalized biomimetic matrices represent a promising strategy for enhancing bone tissue regeneration.
- The study highlights the potential of specific protein incorporation to improve the osteoinductive properties of biomaterials.
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