Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells22:06

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

14.0K
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic...
14.0K
Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells06:41

Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells

2.5K
We present a step-by-step protocol for the isolation of long-term hematopoietic stem cells (LT-HSCs) and short-term HSCs (ST-HSCs) using the Hoxb5 reporter...
2.5K
Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)20:38

Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)

24.2K
24.2K
A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells08:45

A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells

11.0K
A standard Western blotting protocol was optimized for analyzing as few as 500 hematopoietic stem or progenitor cells. Optimization involves careful handling of the cell sample, limiting transfers between tubes, and directly lysing the cells in Laemmli sample...
11.0K
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

7.1K
Bulk gene expression measurements cloud individual cell differences in heterogeneous cell populations. Here, we describe a protocol for how single-cell gene expression analysis and index sorting by Florescence Activated Cell Sorting (FACS) can be combined to delineate heterogeneity and immunophenotypically characterize molecularly distinct cell...
7.1K
A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells07:14

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

5.2K
This protocol enables the maintenance of quiescent human hematopoietic stem cells in vitro by mimicking the bone marrow microenvironment using abundant fatty acids, cholesterol, lower concentrations of cytokines, and...
5.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regulatory T Cell Amelioration of Graft-versus-Host Disease following Allogeneic/Xenogeneic Hematopoietic Stem Cell Transplantation Using Mobilized Mouse and Human Peripheral Blood Donors.

Transplantation and cellular therapy·2023
Same author

Rapamycin targets several pathophysiological features of immune-mediated bone marrow failure in murine models.

Haematologica·2017
Same author

Dynamic equilibrium of heterogeneous and interconvertible multipotent hematopoietic cell subsets.

Scientific reports·2014
Same author

New therapeutic approaches for protecting hematopoietic stem cells in aplastic anemia.

Immunologic research·2013
Same author

Heterogeneity, self-renewal, and differentiation of hematopoietic stem cells.

Stem cells international·2012
Same author

Hematopoietic progenitor cell regulation by CD4+CD25+ T cells.

Blood·2010

Related Experiment Video

Updated: Jan 20, 2026

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
22:06

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Published on: February 25, 2007

14.0K

Hematopoietic Stem Cell Heterogeneity.

Roland Jurecic1

  • 1Department of Microbiology & Immunology, Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, Miami, FL, USA. rjurecic@med.miami.edu.

Advances in Experimental Medicine and Biology
|September 6, 2019
PubMed
Summary
This summary is machine-generated.

Hematopoietic stem cells (HSCs) exhibit diverse subsets with unique functions. Understanding this heterogeneity is key for regenerative medicine and aging research.

Keywords:
Hematopoietic stem cellsHematopoiesisHSC subsetsFunctional heterogeneityDifferentiation bias

More Related Videos

Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells
06:41

Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells

Published on: May 19, 2023

2.5K
Isolation and Transplantation of Hematopoietic Stem Cells HSCs
20:38

Isolation and Transplantation of Hematopoietic Stem Cells HSCs

Published on: February 25, 2007

24.2K

Related Experiment Videos

Last Updated: Jan 20, 2026

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
22:06

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Published on: February 25, 2007

14.0K
Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells
06:41

Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells

Published on: May 19, 2023

2.5K
Isolation and Transplantation of Hematopoietic Stem Cells HSCs
20:38

Isolation and Transplantation of Hematopoietic Stem Cells HSCs

Published on: February 25, 2007

24.2K

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Immunophenotyping

Background:

  • Hematopoietic stem cells (HSCs) are crucial for continuous blood cell production and regeneration.
  • Recent advances reveal HSC heterogeneity, with distinct subsets possessing varied self-renewal and differentiation capabilities.
  • This diversity impacts steady-state, emergency hematopoiesis, and aging processes.

Purpose of the Study:

  • To summarize the current knowledge on phenotypic and functional heterogeneity within the HSC pool.
  • To emphasize the immunophenotypes and functional characteristics of identified HSC subsets.
  • To explore the roles of these subsets in different physiological and pathological conditions.

Main Methods:

  • Utilizing expanded cell surface marker panels for detailed immunophenotyping.
  • Employing advanced functional analyses to assess HSC self-renewal and repopulating capacity.
  • Reviewing existing literature on HSC subset behavior in various hematopoiesis contexts.

Main Results:

  • Identification of distinct HSC subsets based on immunophenotype.
  • Demonstration of varied self-renewal, repopulating potential, and lineage bias among HSC subsets.
  • Elucidation of differential roles of HSC subsets in steady-state, emergency hematopoiesis, and aging.

Conclusions:

  • HSC heterogeneity is a fundamental aspect of hematopoiesis.
  • Understanding specific HSC subset functions is critical for therapeutic applications.
  • Further research is needed to fully elucidate HSC biology in health and disease.