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Embryo Transfer Surgery.

Melissa A Larson1,2

  • 1Transgenic and Gene-Targeting Facility, University of Kansas Medical Center, Kansas City, KS, USA. mlarson@kumc.edu.

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|September 13, 2019
PubMed
Summary
This summary is machine-generated.

Surgical embryo transfer is crucial for creating gene-modified mice and for assisted reproduction techniques. This procedure involves surgically placing manipulated embryos into a pseudopregnant recipient mouse for development.

Keywords:
Embryo transferMousePseudopregnantRecipientRederivationSurgeryVasectomized male

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Area of Science:

  • * Reproductive biology
  • * Genetics and genomics
  • * Laboratory animal science

Background:

  • * Generating gene-modified mice is essential for research, requiring efficient methods for embryo manipulation and transfer.
  • * Assisted reproductive techniques like rederivation and in vitro fertilization also rely on successful embryo transfer.
  • * The transfer of manipulated embryos into a pseudopregnant recipient mouse is a critical step for successful gestation.

Purpose of the Study:

  • * To describe the surgical procedure for transferring embryos into recipient female mice.
  • * To provide guidance on embryo transfer techniques for gene modification and assisted reproduction.

Main Methods:

  • * Surgical transfer of embryos into the oviduct or uterine horn of a pseudopregnant recipient mouse.
  • * Consideration of embryo developmental stage and recipient's reproductive cycle for optimal transfer placement.
  • * Detailed description of the surgical process for embryo transplantation.

Main Results:

  • * Successful development of transferred embryos to term in recipient mice.
  • * Demonstration of the feasibility of surgical embryo transfer for various applications.

Conclusions:

  • * Surgical embryo transfer is a fundamental technique in mouse genetics and reproductive technologies.
  • * The described method ensures the successful development of manipulated embryos, facilitating research and breeding programs.