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Related Concept Videos

Introduction to Innate and Adaptive Immunity01:21

Introduction to Innate and Adaptive Immunity

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The human immune system is a complex defense mechanism that protects the body from harmful pathogens and foreign substances. It comprises two crucial components: innate and adaptive immunity.
Innate immunity is the body's natural, nonspecific defense system that acts quickly to protect against pathogens. It incorporates physical barriers like skin and mucous membranes and cellular elements such as phagocytes and natural killer cells. This part of our immune system provides an immediate,...
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An In Vitro Method to Identify Innate and Adaptive Immune Cells Residing in Murine Lungs05:54

An In Vitro Method to Identify Innate and Adaptive Immune Cells Residing in Murine Lungs

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This video demonstrates an in vitro technique to identify immune cells residing in murine lungs. After isolating target immune cells from murine lung tissue, they are surface-labeled using a cocktail of fluorophore-tagged antibodies against specific markers on innate and adaptive immune cells. Additionally, monocytes, macrophages, and dendritic cells are labeled using an intracellular marker. The various immune cell types are then identified using flow...
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Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung09:57

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

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In this study, we present an effective and reproducible protocol to isolate the immune populations of the murine respiratory system. We also provide a method for the identification of all innate and adaptive immune cells that reside in the lungs of healthy mice, using a 9-color-based flow cytometry...
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Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

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The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
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Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages12:47

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

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In this protocol, we describe methods to efficiently transfect murine macrophage cell lines with siRNAs using the Amaxa Nucleofector 96-well Shuttle System, stimulate the macrophages with lipopolysaccharide, and monitor the effects on inflammatory cytokine...
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Related Experiment Video

Updated: Jan 19, 2026

Introduction to Innate and Adaptive Immunity
01:21

Introduction to Innate and Adaptive Immunity

8.9K

γδ T Cells Promote Steatohepatitis by Orchestrating Innate and Adaptive Immune Programming.

Alejandro Torres-Hernandez1, Wei Wang1, Yuri Nikiforov1

  • 1Department of Surgery, S.A. Localio Laboratory, New York University School of Medicine, New York, NY.

Hepatology (Baltimore, Md.)
|September 19, 2019
PubMed
Summary
This summary is machine-generated.

Gamma delta T (γδT) cells exacerbate nonalcoholic steatohepatitis (NASH) by impairing CD4+ T cell expansion. Targeting γδT cell recruitment protects against and resolves diet-induced NASH.

More Related Videos

An In Vitro Method to Identify Innate and Adaptive Immune Cells Residing in Murine Lungs
05:54

An In Vitro Method to Identify Innate and Adaptive Immune Cells Residing in Murine Lungs

552
Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung
09:57

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

Published on: November 16, 2021

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Related Experiment Videos

Last Updated: Jan 19, 2026

Introduction to Innate and Adaptive Immunity
01:21

Introduction to Innate and Adaptive Immunity

8.9K
An In Vitro Method to Identify Innate and Adaptive Immune Cells Residing in Murine Lungs
05:54

An In Vitro Method to Identify Innate and Adaptive Immune Cells Residing in Murine Lungs

552
Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung
09:57

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

Published on: November 16, 2021

7.7K

Area of Science:

  • Immunology
  • Hepatology
  • Gastroenterology

Background:

  • Inflammatory cell recruitment drives the progression from hepatic steatosis to steatohepatitis (SH).
  • Understanding the specific immune cells involved is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the role of gamma delta T (γδT) cells in the pathogenesis of steatohepatitis (SH).
  • To elucidate the mechanisms by which γδT cells contribute to SH progression.
  • To evaluate the therapeutic potential of targeting γδT cells in SH.

Main Methods:

  • Analysis of γδT cell recruitment and phenotype in SH models.
  • Investigation of signaling pathways (CCR2, CCR5, NOD2, ICOS) involved in γδT cell activation.
  • Assessment of γδT cell interaction with lipid antigens (palmitic acid) and CD1d.
  • Evaluation of the impact of γδT cell depletion or recruitment inhibition on diet-induced SH.

Main Results:

  • γδT cells are recruited to the liver in SH via CCR2, CCR5, and NOD2 signaling.
  • γδT cells adopt an IL-17A+ phenotype dependent on ICOS/ICOS ligand.
  • γδT cells upregulate CD1d and CD36, presenting lipid antigens and responding to palmitic acid.
  • Deletion or depletion of γδT cells ameliorates diet-induced SH and promotes resolution.

Conclusions:

  • Hepatic γδT cells exacerbate SH independently of IL-17A.
  • γδT cells impair conventional CD4+ T cell expansion and modulate their inflammatory program via CD1d-dependent VEGF.
  • Targeting γδT cell recruitment offers a potential therapeutic strategy for SH.