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Related Experiment Videos

Steroid profiling--an update.

N A Schmidt, H J Borburgh, T J Penders

    Clinical Chemistry
    |April 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    To improve steroid analysis, use an amino (NH2) column with C18 cartridges for cleaner urine extracts. Pre-hydrolysis extraction is crucial for optimal recovery of dehydroepiandrosterone, avoiding excessive enzyme use.

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    Area of Science:

    • Endocrinology
    • Analytical Chemistry
    • Biochemistry

    Background:

    • Urine steroid extraction using C18 cartridges often yields impure samples.
    • Impurities can interfere with subsequent analyses, such as gas chromatography.

    Purpose of the Study:

    • To develop a cleaner method for extracting steroids from urine.
    • To optimize the analytical recovery of dehydroepiandrosterone (DHEA).

    Main Methods:

    • Solid-phase extraction (SPE) using C18 cartridges.
    • Sequential SPE using C18 and amino (NH2) columns.
    • Hydrolysis of steroid conjugates using Helix pomatia enzyme preparation.

    Main Results:

    • Employing an NH2 column in series with a C18 column significantly reduces impurities in urine steroid extracts.

    Related Experiment Videos

  • Increasing amounts of Helix pomatia enzyme preparation negatively impact the analytical recovery of dehydroepiandrosterone.
  • Extracting steroid conjugates with C18 columns prior to hydrolysis is essential for maximizing DHEA recovery.

    • Conclusions:

    • A sequential C18 and NH2 column extraction method provides a sufficiently clean sample for gas-chromatographic analysis of urinary steroids.
    • Optimizing the amount of enzyme preparation during hydrolysis is critical to balance complete conjugate splitting and maintain high analytical recovery of DHEA.