Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.8K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phasor EO-FLIM: Lifetime imaging with picosecond noise and 500 Hz frame rate.

bioRxiv : the preprint server for biology·2026
Same author

Fast wide-field light sheet electro-optic FLIM.

Optics express·2026
Same author

Low-Energy Single-Electron Detector with Submicron Resolution.

ACS photonics·2026
Same author

Optimizing the localization precision in coherent scattering microscopy using structured light.

Nanophotonics (Berlin, Germany)·2025
Same author

Wide-field fluorescence lifetime imaging of neuron spiking and subthreshold activity in vivo.

Science (New York, N.Y.)·2023
Same author

Distributed quantum sensing with mode-entangled spin-squeezed atomic states.

Nature·2022
Same journal

The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells.

Nature communications·2026
Same journal

Signaling downstream of tumor-stroma interaction regulates mucinous colorectal adenocarcinoma apicobasal polarity.

Nature communications·2026
Same journal

Click-polymerized polyenamine membranes for efficient lithium extraction.

Nature communications·2026
Same journal

Joint trajectories of brain atrophy, white matter hyperintensities and cognition quantify brain maintenance.

Nature communications·2026
Same journal

Proton shuttling at electrochemical interfaces under alkaline hydrogen evolution.

Nature communications·2026
Same journal

metilene<sup>3</sup>: identifying DMRs across multiple conditions with auto-classification.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Jan 6, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

25.8K

Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy.

Adam J Bowman1, Brannon B Klopfer2, Thomas Juffmann3,4

  • 1Physics Department, Stanford University, 382 Via Pueblo Mall, Stanford, CA, 94305, USA. abowman2@stanford.edu.

Nature Communications
|October 10, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed a Pockels cell technique for high-efficiency, nanosecond-resolution wide-field imaging. This method enhances fluorescence lifetime imaging microscopy (FLIM) and single-molecule spectroscopy, improving throughput for low-light applications.

More Related Videos

Fluorescence Lifetime Macro Imager for Biomedical Applications
06:01

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

1.1K
Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System
08:35

Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

Published on: December 16, 2019

9.7K

Related Experiment Videos

Last Updated: Jan 6, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

25.8K
Fluorescence Lifetime Macro Imager for Biomedical Applications
06:01

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

1.1K
Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System
08:35

Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

Published on: December 16, 2019

9.7K

Area of Science:

  • Optics and Photonics
  • Biophotonics
  • Microscopy

Background:

  • Nanosecond temporal resolution is crucial for advanced wide-field imaging techniques.
  • Existing methods struggle with optical efficiency, limiting low-light applications like fluorescence microscopy.
  • Pockels cells offer potential for high-speed optical gating.

Purpose of the Study:

  • To demonstrate Pockels cells for efficient wide-field image gating with nanosecond resolution.
  • To enhance throughput for fluorescence lifetime imaging microscopy (FLIM) and single-molecule spectroscopy.
  • To enable nanosecond imaging with standard optical systems for low-light microscopy.

Main Methods:

  • Utilizing Pockels cells with polarizing beam-splitters to capture two temporal frames.
  • Implementing multi-label FLIM, single-molecule lifetime spectroscopy, and fast single-frame FLIM.
  • Developing a space-to-time image multiplexer with a re-imaging optical cavity and tilted mirror.

Main Results:

  • Achieved nanosecond temporal resolution and high photon collection efficiency in wide-field imaging.
  • Demonstrated significant throughput increase (10^3-10^5 times) compared to single photon counting.
  • Enabled advanced FLIM and single-molecule spectroscopy techniques with enhanced speed and efficiency.

Conclusions:

  • Pockels cells provide an efficient solution for nanosecond-resolution wide-field imaging.
  • The developed methods significantly improve throughput for low-light microscopy applications.
  • This technique opens new possibilities for temporal dimension imaging in microscopy.