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Related Concept Videos

Subviral Agents01:29

Subviral Agents

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Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
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Viruses are unique biological entities that blur the boundary between living and non-living systems. Although they lack cellular structure and metabolic processes, they can exhibit characteristics of life when infecting a host. Their defining feature is a nucleic acid core, composed of either DNA or RNA, encapsulated within a protein coat called a capsid. This simple structure allows them to invade host cells and use their machinery for replication efficiently.Viral Structure and...
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Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods
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Virus-Derived Nanoparticles.

Noor H Dashti1, Frank Sainsbury2,3

  • 1Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|October 16, 2019
PubMed
Summary
This summary is machine-generated.

This study details the purification, characterization, and chemical labeling of virus-derived nanoparticles. These viral nanoparticles offer versatile applications in nanotechnology, including therapeutic delivery and nanoreactors.

Keywords:
Density gradientIntracellular uptakeParticle size distributionViral nanoparticleVirus-like nanoparticle

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Area of Science:

  • Nanotechnology
  • Biotechnology
  • Virology

Background:

  • Capsid-based virus particles are engineered into viral nanoparticles and virus-like nanoparticles.
  • Their organized structures are suitable for in vitro and in vivo applications like therapeutic delivery and enzymatic nanoreactors.
  • Viruses can be produced in diverse expression systems, from bacteria to plants.

Purpose of the Study:

  • To provide a generic protocol for purifying and characterizing virus-derived nanoparticles.
  • To present a methodology for chemically labeling these nanoparticles.
  • To enable monitoring of nanoparticle uptake in mammalian cells.

Main Methods:

  • Purification of virus-derived nanoparticles.
  • Characterization of nanoparticle structure and uniformity.
  • Chemical labeling techniques for nanoparticle tracking.
  • Mammalian cell uptake studies.

Main Results:

  • Established protocols for producing and purifying viral nanoparticles.
  • Demonstrated successful chemical labeling for cellular monitoring.
  • Validated the utility of these nanoparticles in biological systems.

Conclusions:

  • Virus-derived nanoparticles are highly adaptable for nanotechnology applications.
  • Customizable viral capsids offer significant potential in therapeutic delivery and nanoreactor design.
  • The presented methods facilitate the use of viral nanoparticles in biomedical research.