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Expression, Purification, and Crystallization of Full-Length HSV-1 gB for Structure Determination
Rebecca S Cooper1, Ekaterina E Heldwein2
1Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 17, 2019
Abstract
None:
HSV glycoproteins play important roles in the viral life cycle, particularly viral cell entry. Here we describe the protocol for expression, purification, and crystallization of full-length HSV-1 glycoprotein B. The protocol provides a framework for incorporating transmembrane domain-stabilizing amphipols into the crystallization setup and can be adapted to isolate other complete HSV glycoproteins.
