Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deficits in motivated behavior in a mouse model of paclitaxel-induced neuropathic pain.

Physiology & behavior·2026
Same author

Factors influencing the effectiveness of case management interventions for suicide attempters in a psychiatric hospital.

PCN reports : psychiatry and clinical neurosciences·2025
Same author

Three-dimensional markerless motion capture of multiple freely behaving monkeys toward automated characterization of social behavior.

Science advances·2025
Same author

Role of the monkey orbitofrontal cortex in processing the choice history during reward-based decision-making.

Cerebral cortex (New York, N.Y. : 1991)·2025
Same author

Chemogenetic Disruption of Monkey Perirhinal Neurons Projecting to the Rostromedial Caudate Impairs Associative Learning.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2025
Same author

Detectability in Scalp EEGs of Epileptic Spikes Emitted from Brain Electrical Sources of Different Sizes and Locations: A Simulation Study Using Realistic Head Models of Elderly Adults.

Clinical EEG and neuroscience·2025
Same journal

Modulation of the EEG alpha-band envelope during mental tasks: A task-related index based on second-order derivatives.

Neuroscience research·2026
Same journal

Enhanced calcium activity and transcriptomic alterations in iPSC-derived neurons from BAFME patients with repeat expansions.

Neuroscience research·2026
Same journal

Clustered protocadherinβs contribute to configuring functional cell populations in hippocampal-cortical regions.

Neuroscience research·2026
Same journal

ALS-associated protein TDP-43 disturbs axonal projections in the somatosensory cortex.

Neuroscience research·2026
Same journal

Microglial ontogeny and in vitro reconstruction: Bridging development and modeling.

Neuroscience research·2026
Same journal

A layer specific histological framework for synaptic quantification in hippocampal CA1.

Neuroscience research·2026
See all related articles

Related Experiment Video

Updated: Jan 5, 2026

Recording Network Activity in Spinal Nociceptive Circuits Using Microelectrode Arrays
11:28

Recording Network Activity in Spinal Nociceptive Circuits Using Microelectrode Arrays

Published on: February 9, 2022

3.3K

Reverse-filtering on extracellular action potential for waveform analysis.

Takashi Mizuhiki1, Tsuyoshi Setogawa1, Munetaka Shidara1

  • 1Faculty of Medicine, University of Tsukuba, 1-1-1 Ten-nodai, Tsukuba, Ibaraki 305-8577, Japan.

Neuroscience Research
|October 19, 2019
PubMed
Summary
This summary is machine-generated.

Identifying serotonergic neurons using extracellular action potential (EAP) duration is inaccurate. Restored EAP waveforms suggest dorsal raphe neurons may not be exclusively serotonergic, requiring reevaluation of conventional criteria.

Keywords:
AveragingDeconvolutionExtracellular action potentialFourier transformMonkeyReverse-FilteringSerotoninWaveform

More Related Videos

High Density Event-related Potential Data Acquisition in Cognitive Neuroscience
08:33

High Density Event-related Potential Data Acquisition in Cognitive Neuroscience

Published on: April 16, 2010

13.0K
Interfacing Microfluidics with Microelectrode Arrays for Studying Neuronal Communication and Axonal Signal Propagation
11:27

Interfacing Microfluidics with Microelectrode Arrays for Studying Neuronal Communication and Axonal Signal Propagation

Published on: December 8, 2018

8.5K

Related Experiment Videos

Last Updated: Jan 5, 2026

Recording Network Activity in Spinal Nociceptive Circuits Using Microelectrode Arrays
11:28

Recording Network Activity in Spinal Nociceptive Circuits Using Microelectrode Arrays

Published on: February 9, 2022

3.3K
High Density Event-related Potential Data Acquisition in Cognitive Neuroscience
08:33

High Density Event-related Potential Data Acquisition in Cognitive Neuroscience

Published on: April 16, 2010

13.0K
Interfacing Microfluidics with Microelectrode Arrays for Studying Neuronal Communication and Axonal Signal Propagation
11:27

Interfacing Microfluidics with Microelectrode Arrays for Studying Neuronal Communication and Axonal Signal Propagation

Published on: December 8, 2018

8.5K

Area of Science:

  • Neuroscience
  • Computational Neuroscience

Background:

  • Serotonergic neurons are crucial for understanding brain function, necessitating accurate identification methods.
  • Current methods using extracellular action potential (EAP) duration for identifying serotonergic neurons are limited by signal filtering.
  • Passband limitations in spike sorting can distort EAP duration, impacting neuron classification.

Purpose of the Study:

  • To develop and validate methods for restoring original extracellular action potential (EAP) waveforms.
  • To re-evaluate the criteria for identifying serotonergic neurons in the dorsal raphe (DR) nucleus.
  • To compare EAP characteristics between the dorsal raphe (DR) and orbitofrontal cortex (OFC).

Main Methods:

  • Averaging EAPs from unfiltered raw signals to reconstruct original waveforms.
  • Reverse-filtering EAPs from filtered raw signals to restore original waveforms.
  • Applying multivariate analyses to reconstructed EAPs from DR and OFC.

Main Results:

  • Reconstructed EAP waveforms using averaging and reverse-filtering showed high agreement.
  • Multivariate analysis of reconstructed DR EAPs did not yield distinct clusters, suggesting mixed neuron populations.
  • While filtered EAP durations showed no difference between DR and OFC, restored EAPs revealed shorter first crest durations in DR compared to OFC.

Conclusions:

  • Both averaging and reverse-filtering are effective for restoring EAP waveforms.
  • The conventional waveform criterion for identifying serotonergic neurons in the DR may be unreliable, potentially including non-serotonergic neurons.
  • Differences in restored EAP waveforms between DR and OFC suggest a need to reconsider current identification methods for serotonergic neurons.