Combining gold nanoparticle-based headspace single-drop microextraction and a paper-based colorimetric assay for...

Area of Science:

• Analytical Chemistry
• Nanotechnology
• Environmental Science

Background:

• Selenium(IV) (Se(IV)) determination is crucial for environmental and health monitoring.
• Existing methods for Se(IV) detection can be complex and time-consuming.
• Development of rapid, sensitive, and selective analytical techniques is needed.

Purpose of the Study:

• To develop a novel, sensitive, and selective method for Se(IV) determination.
• To combine headspace single-drop microextraction with a paper-based colorimetric assay.
• To utilize unmodified gold nanoparticles (AuNPs) as both extractant and colorimetric probe.

Main Methods:

• Headspace single-drop microextraction with AuNPs for Se(IV) extraction.
• Colorimetric detection based on hydrogen selenide adsorption onto AuNPs.
• Scanometric-assisted digital image analysis for quantification on cellulose paper.

Main Results:

• Optimal analytical conditions were determined for sensitivity.
• A linear correlation was achieved for Se(IV) concentrations from 15-100 μg L⁻¹.
• A low limit of quantification (12 μg L⁻¹) and good precision (RSD < 7%) were obtained.
• Satisfactory results were achieved for real sample analysis.

Conclusions:

• The developed method offers a sensitive, selective, and efficient approach for Se(IV) determination.
• The combination of microextraction, AuNPs, and paper-based assay provides a cost-effective analytical tool.
• This method shows potential for practical application in environmental and biological sample analysis.