Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.9K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.9K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

11.0K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
11.0K
Interference and Diffraction02:18

Interference and Diffraction

51.5K
Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
51.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondria directly interact with the nuclear pore complex.

Nature·2026
Same author

Membrane-separated electrodes enable high-rate low-energy electrochemical carbon capture.

Science advances·2026
Same author

Protein Response to ACL Injury in Humans Show Early Cartilage Remodeling and Differences by Sex.

bioRxiv : the preprint server for biology·2026
Same author

The Transcription Factor 12 of Basic Helix-Loop-Helix Plays an Essential Role in Retinal Health.

Investigative ophthalmology & visual science·2026
Same author

Linkage between HLA-B8 and HLA-DQ2.5 Contributes to Ancestry-Dependent Genetic Risk for Celiac Disease.

medRxiv : the preprint server for health sciences·2026
Same author

Covalently sewing dual-sided Janus coatings <i>via</i> photoiniferter surface chain extension.

Materials horizons·2026
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Jan 4, 2026

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
11:34

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

16.0K

Micro-Frequency Shifting Projection Technique for Inter-Reflection Removal.

Zhaoshuai Qi, Zhao Wang, Junhui Huang

    Optics Express
    |November 6, 2019
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a micro-frequency shifting (MFS) technique to remove inter-reflections from complex scenes. The method effectively handles both diffuse and specular reflections, improving 3D reconstruction accuracy.

    More Related Videos

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    8.8K
    Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
    06:43

    Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

    Published on: May 3, 2022

    3.9K

    Related Experiment Videos

    Last Updated: Jan 4, 2026

    High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
    11:34

    High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

    Published on: December 3, 2013

    16.0K
    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    8.8K
    Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
    06:43

    Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

    Published on: May 3, 2022

    3.9K

    Area of Science:

    • Computer Vision
    • 3D Reconstruction
    • Optical Metrology

    Background:

    • Inter-reflection removal is crucial for accurate 3D scene reconstruction.
    • Existing methods often fail with specular surfaces, limiting their application.
    • Diffuse-only inter-reflection removal is insufficient for complex real-world scenes.

    Purpose of the Study:

    • To develop a novel technique for removing both diffuse and specular inter-reflections.
    • To enhance the accuracy and robustness of 3D reconstruction in complex scenes.
    • To address limitations of current inter-reflection removal methods.

    Main Methods:

    • A micro-frequency shifting (MFS) projection technique is proposed.
    • Patterns with specific frequency shifts and base frequencies are projected.
    • Inter-reflections are detected by analyzing modulation variations and removed using regional projection.

    Main Results:

    • The MFS technique successfully detects inter-reflections caused by both diffuse and specular surfaces.
    • Experimental results demonstrate effective removal of complex inter-reflections.
    • The proposed method significantly improves the quality of 3D scene reconstruction.

    Conclusions:

    • The MFS projection technique offers a robust solution for inter-reflection removal in complex scenes.
    • This method broadens the applicability of 3D reconstruction to surfaces with both diffuse and specular properties.
    • The findings pave the way for more accurate and versatile 3D reconstruction systems.