Hadamard acquisition of ¹³ C-¹³ C 2-D correlation NMR spectra

We show that a multiselective excitation with Hadamard encoding is a powerful tool for 2-D acquisition of ¹³ C─¹³ C homonuclear correlations. This method is not designed to improve the sensitivity, but rather to reduce the experiment time, provided there is sufficient sensitivity. Therefore, it allows fast acquisition of such 2-D spectra in labeled molecules. The technique has been demonstrated using a U─¹³ C─¹⁵ N histidine hydrochloride monohydrate sample allowing each point of the build-up curves of the ¹³ C─¹³ C cross-peaks to be recorded within 4 min 35 s, which is very difficult with conventional methods. Using the U─¹³ C─¹⁵ N f-MLF sample, we have demonstrated that the method can be applied to molecules with 14 ¹³ C resonances with a minimum frequency separation of 240 Hz.