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Updated: Jan 2, 2026

Fluorescence Lifetime Macro Imager for Biomedical Applications

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

Fiber Optic pH Sensor Based on Phase Fluorescence Lifetimes.

Richard B Thompson¹, Joseph R Lakowicz¹

¹Center for Fluorescence Spectroscopy and Department of Biological Chemistry, School of Medicine, University of Maryland, 108 North Greene Street, Baltimore, Maryland 21201.

Analytical Chemistry

|December 14, 2019

Summary

This summary is machine-generated.

A novel fiber optic pH sensor uses fluorescence lifetime measurements for high accuracy. This adaptable method offers precise pH readings (0.02 units) and can be extended to other analytes.

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Area of Science:

Analytical Chemistry
Biomedical Engineering
Optoelectronics

Background:

Accurate pH monitoring is crucial in various scientific and industrial applications.
Existing pH sensing technologies have limitations in certain environments.
Fiber optic sensing offers advantages in remote and harsh conditions.

Purpose of the Study:

To develop a novel fiber optic pH sensor.
To utilize phase fluorescence lifetime measurements for pH sensing.
To achieve high accuracy and precision in pH measurements.

Main Methods:

Employed single fiber phase fluorescence lifetime measurements.
Used commercially available fluorescence indicators.
Modified an existing phase fluorometer apparatus.

Main Results:

Achieved accuracy and precision of approximately 0.02 pH units.
Demonstrated a straightforward modification of existing equipment.
Validated the sensor's performance for pH determination.

Conclusions:

The developed fiber optic sensor provides a highly accurate and precise method for pH measurement.
The approach is versatile and applicable to sensing other analytes.
Potential for integration into evanescent wave sensing schemes exists.