Related Experiment Video
Updated: Jan 1, 2026

Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy
Published on: December 15, 2011
Metabolic Profiling and Untargeted 1H-NMR-Based Metabolomics Study of Different Iranian Pomegranate (Punica granatum)
Maede Hasanpour1, Satar Saberi2, Mehrdad Iranshahi1
1Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Abstract:
Pomegranate (Punica granatum) is an ancient fruit that is widely consumed as fresh fruit and juice. The aim of the present study was to compare the metabolic profile of pomegranate ecotypes from different geographical origins of Iran, the largest producer of pomegranates in the world. 1H-NMR and 2D NMR spectroscopy were applied to investigate the ecotypic variation. Multivariate data analyses were used to identify overall metabolic differences. Mazandaran pomegranate samples were found to be different from the other ecotypes, having a high content of citric and succinic acids. Bajestan, Ferdows, and Yazd pomegranates contained comparatively higher amounts of anthocyanins and ellagic acid derivatives than other pomegranate ecotypes. The distribution of metabolites among different ecotypes of pomegranate is discussed on the basis of these findings.

