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Direct Surface Enhanced Raman Spectroscopic Detection of Cortisol at Physiological Concentrations
T Joshua Moore1, Bhavya Sharma1
1Department of Chemistry , The University of Tennessee Knoxville , 1420 Circle Drive , Knoxville , Tennessee 37996 , United States.
Analytical Chemistry
|December 25, 2019
Summary
Surface-enhanced Raman spectroscopy (SERS) offers a novel method for detecting cortisol, a key stress hormone. This technique shows promise for improved diagnostics of cortisol-related conditions.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Spectroscopy
Background:
- Cortisol is a vital steroid hormone regulating stress response.
- Abnormal cortisol levels are linked to Addison's and Cushing syndromes.
- Current cortisol detection methods are time-consuming and destructive.
Purpose of the Study:
- To develop a rapid and sensitive method for cortisol detection.
- To utilize Surface-Enhanced Raman Spectroscopy (SERS) for cortisol analysis.
- To establish SERS as a viable technique for monitoring physiological cortisol levels.
Main Methods:
- Employing colloidal silver nanoparticles to enhance Raman signals.
- Analyzing cortisol in ethanolic solutions and complex biological matrices (bovine serum albumin).
- Utilizing principal components analysis for data interpretation in complex samples.
Main Results:
- Demonstrated SERS detection of cortisol within the physiologically relevant range.
- Established a limit of detection at 177 nM in an ethanol matrix.
- Showcased linear separation of cortisol concentrations in complex matrices using principal components analysis.
Conclusions:
- SERS provides a sensitive and rapid method for cortisol detection.
- The developed SERS technique holds potential for improved clinical diagnostics.
- Multivariate analysis enhances the utility of SERS for complex biological samples.
