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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Dec 31, 2025

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Published on: July 21, 2018

Quantitative Surface Plasmon Interferometry via Upconversion Photoluminescence Mapping.

Anxiang Yin^1,2, Hao Jing¹, Zhan Wu^3,4

¹Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA.

Research (Washington, D.C.)

|January 11, 2020

Summary

This summary is machine-generated.

Rare-earth-doped nanoparticles visualize surface plasmon polaritons (SPPs) in real-time. This method enables ultrasensitive, label-free detection of biomolecules by converting SPPs into visible light signals.

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Last Updated: Dec 31, 2025

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Published on: June 23, 2022

Area of Science:

Plasmonics
Nanotechnology
Biophotonics

Background:

Surface plasmon polaritons (SPPs) are crucial for optics and materials science.
Current near-field techniques for SPP visualization are complex and not suitable for real-time applications.
Developing direct far-field methods for SPP characterization is essential for advanced applications.

Purpose of the Study:

To develop a novel method for direct far-field visualization and characterization of SPPs.
To enable real-time, high-throughput detection of SPPs in complex environments.
To create a sensitive platform for label-free biomolecule detection.

Main Methods:

Utilized rare-earth-doped nanoparticles to upconvert SPPs into visible photoluminescence.
Employed interference fringes between SPPs and incident light for quantitative analysis.
Developed a spectrometer-free optical ruler based on SPP interference patterns.

Main Results:

Achieved direct far-field visualization of SPPs in complicated environments.
Quantitatively measured SPP wavelength, propagation length, and local dielectric environments.
Demonstrated ultrasensitive, label-free detection of biomolecules (e.g., streptavidin, PSA) down to femtomolar levels.

Conclusions:

Rare-earth-doped nanoparticles provide a powerful tool for SPP visualization and characterization.
The developed method offers a new signaling pathway for sensitive detection of dielectric environment changes.
This technology enables rapid, spectrometer-free optical rulers for diverse biosensing applications.