Related Experiment Video
Updated: Dec 30, 2025
12:49
Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry
Published on: April 4, 2018
12.0K
Inspector: a lysine succinylation predictor based on edited nearest-neighbor undersampling and adaptive synthetic
Yan Zhu1, Cangzhi Jia1, Fuyi Li2
1School of Science, Dalian Maritime University, Dalian, 116026, China.
Analytical Biochemistry
|January 23, 2020
Summary
Identifying lysine succinylation sites is crucial for understanding protein function. This study introduces Inspector, a novel computational tool that accurately predicts these sites using sequence data, overcoming limitations of traditional methods.
Area of Science:
- Biochemistry
- Proteomics
- Bioinformatics
Background:
- Lysine succinylation is a key post-translational modification regulating protein function.
- The precise mechanisms and functions of succinylation remain unclear.
- Identifying lysine succinylation sites is essential for deeper understanding.
Purpose of the Study:
- To develop an effective computational approach for identifying lysine succinylation sites.
- To overcome the limitations of expensive and time-consuming experimental methods.
- To introduce a novel predictor named Inspector.
Main Methods:
- Employed the random forest algorithm for predictor development.
- Utilized various sequence-based feature-encoding schemes.
- Applied undersampling and oversampling techniques to address dataset imbalance.
- Implemented a two-step feature-selection strategy for model optimization.
Main Results:
- Developed a novel predictor, Inspector, for lysine succinylation site identification.
- Achieved competitive predictive performance compared to existing tools.
- Demonstrated the effectiveness of the random forest algorithm and feature selection strategies.
Conclusions:
- Inspector provides an efficient and accurate method for identifying lysine succinylation sites from sequence data.
- The developed computational approach aids in understanding the functional roles of protein succinylation.
- This tool can accelerate research in post-translational modification studies.
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