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A Method for Islet Transplantation to the Omentum in Mouse
Published on: January 7, 2019
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Pig-to-Macaque Islet Xenotransplantation
Suzanne Bertera1, Michael F Knoll1, Carmela A Knoll1
1Institute of Cellular Therapeutics, Allegheny Health Network Research Institute, Allegheny Health Network, Pittsburgh, PA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|February 1, 2020
Summary
This chapter details successful methods for isolating porcine islets and transplanting them into non-human primates (NHPs) to treat diabetes. These procedures are crucial for advancing clinical applications of pig islet xenotransplantation in humans.
Area of Science:
- Xenotransplantation research
- Diabetes mellitus treatment
- Primate models in research
Background:
- Clinical application of porcine islets for diabetes cure requires reproducible success in non-human primate (NHP) models.
- Significant global research efforts are dedicated to achieving this goal.
Purpose of the Study:
- To outline established procedures for successful pig-to-NHP islet transplantation.
- To provide a comprehensive guide for researchers in the field of islet xenotransplantation.
Main Methods:
- Detailed protocols for isolating pancreatic islets from adult and neonatal/fetal pigs.
- Procedures for inducing diabetes in NHPs.
- Methods for insulin therapy, islet transplantation, and immunosuppression in xenotransplanted NHPs.
- Guidelines for the daily maintenance of xenotransplanted NHPs.
Main Results:
- The chapter describes procedures successfully utilized in pig-to-NHP islet transplantation models.
- Alternative methods are identified where appropriate, offering flexibility for researchers.
Conclusions:
- The outlined procedures represent a validated approach for pig islet xenotransplantation in NHP models.
- Successful implementation of these methods is critical for advancing toward clinical applications of porcine islets for human diabetes treatment.
