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Using Caco-2 Cells to Study Lipid Transport by the Intestine
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Novel milk casein-derived peptides decrease cholesterol micellar solubility and cholesterol intestinal absorption in

Xiaoxiao Jiang1, Daodong Pan2, Tao Zhang1

  • 1Department of Food Science and Technology, School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 210023 China.

Journal of Dairy Science
|March 2, 2020
PubMed
Summary
This summary is machine-generated.

Milk-derived peptides were identified that reduce cholesterol micellar solubility. These bioactive peptides influence cholesterol absorption-related protein and enzyme expression in intestinal cells, offering a novel approach to cholesterol management.

Keywords:
Caco-2 cellsNPC1L1bioactive peptidescholesterol-lowering solubility of micellarmilk casein

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Area of Science:

  • Biochemistry
  • Nutritional Science
  • Molecular Biology

Background:

  • Milk casein hydrolysis yields bioactive peptides with potential health benefits.
  • Understanding the mechanisms of cholesterol absorption is crucial for managing hypercholesterolemia.

Purpose of the Study:

  • To assess the cholesterol-lowering activity of specific milk casein-derived peptides.
  • To investigate the effects of these peptides on cholesterol absorption-related gene and protein expression in intestinal cells.

Main Methods:

  • Milk casein was hydrolyzed with neutrase, and bioactive peptides were isolated using ultrafiltration and Sephadex G-10 chromatography.
  • Peptide identification was performed using ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry.
  • The impact of peptides on cholesterol solubility, Caco-2 cell protein levels (NPC1L1), and mRNA expression (ABCG8, ABCA1, ACAT2, MTP) was evaluated.

Main Results:

  • Four peptides (TDVEN, LQPE, VAPFPE, VLPVPQ) were identified that reduced micellar cholesterol solubility.
  • LQPE, VLPVPQ, and VAPFPE significantly decreased Niemann-Pick C1-Like 1 (NPC1L1) protein levels.
  • Peptides upregulated ATP binding cassette subfamily G member 8 (ABCG8) and ATP-binding cassette transporter A1 (ABCA1) mRNA expression.
  • VLPVPQ notably decreased acetyl-CoA-acetyltransferase 2 (ACAT2) and microsomal triacylglycerols (MTP) mRNA expression.

Conclusions:

  • Milk-derived peptides can suppress micellar cholesterol solubility.
  • These peptides modulate the expression of key proteins and enzymes involved in intestinal cholesterol absorption.
  • This study provides a theoretical basis for the in vitro cholesterol-lowering effects of novel milk-derived peptides.