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Updated: Jul 15, 2026

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
Published on: February 24, 2015
Using changes in speciation in a dynamic combinatorial library as a fingerprint to differentiate the methylation
Alexandria G Mullins1, Lauren E St Louis, Marcey L Waters
1Department of Chemistry, CB 3290, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. mlwaters@unc.edu.
Abstract:
Herein we describe the development of a sensor array that utilizes the complex response of a dynamic combinatorial library (DCL) to discriminate all of the methylation states of Arg, previously unreported in a sensor array, as well as the methylation states of Lys. We find that the use of all species in the DCL, not just those that bind, allows for discrimination of analytes that are otherwise indistinguishable, demonstrating the value of utilizing a complex network of species for differential sensing.

