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A fluorescence lifetime-based solid sensor for water.

Qing Chang1,2, Zakir Murtaza1,3, Joseph R Lakowicz1,3

  • 1Medical Biotechnology Center, University of Maryland Biotechnology Institute, 725 West Lombard Street, 2nd Floor; Baltimore, MD 21201, USA.

Analytica Chimica Acta
|March 12, 2020
PubMed
Summary
This summary is machine-generated.

A novel fluorescence lifetime-based water sensor utilizes an osmium(II) compound immobilized on a solid film. This sensor accurately detects water content in organic solvents with rapid response and high stability.

Keywords:
FluorescenceLEDLifetime-based sensingSolid state sensor

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Spectroscopy

Background:

  • Solvent-polarity-sensitive fluorescent compounds offer potential for chemical sensing.
  • Metal-ligand complexes, such as osmium(II) compounds, exhibit unique photophysical properties suitable for sensor development.
  • Developing robust solid-state sensors is crucial for practical applications in various environments.

Purpose of the Study:

  • To develop a fluorescence lifetime-based water sensor using a novel osmium(II) metal-ligand compound.
  • To immobilize the fluorescent compound onto a solid support for enhanced sensor fabrication and stability.
  • To evaluate the sensor's performance, including response time, detection limit, and long-term stability in detecting water in organic solvents.

Main Methods:

  • Synthesis and characterization of dipyrido[3,2-a:2″,3″-c]phenazine, di[cis-1,2-bis(diphenylphosphino)-ethylene] osmium(II) hexafluorophosphate ([Os(dppz)(dppe)2](PF6)2).
  • Immobilization of the osmium(II) compound onto carboxymethyl cellulose via ionic bonding.
  • Fabrication of a solid film sensor by sandwiching the immobilized compound between sol-gel and glass layers.
  • Measurement of fluorescence lifetime changes using phase-modulation fluorometry with a blue LED.

Main Results:

  • The immobilized osmium(II) compound exhibited fluorescence quenching in the presence of water, leading to a decrease in fluorescence lifetime.
  • The solid film sensor demonstrated a measurable phase angle decrease proportional to water content, with a 39.6-degree change for 0-20% water in acetone.
  • The sensor achieved a low detection limit of 0.02% (v/v) water in ethyl acetate and showed rapid response/recovery times (few seconds).
  • Excellent long-term stability was observed, with minimal performance degradation after two months.

Conclusions:

  • A highly sensitive and stable fluorescence lifetime-based water sensor was successfully developed.
  • The sensor's ability to detect water in organic solvents via fluorescence lifetime changes offers a promising analytical tool.
  • The solid-state design and rapid response characteristics make this sensor suitable for practical water content monitoring applications.