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Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
Published on: June 8, 2019
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A biosensor based on multifunctional allostery for dynamic analysis of circulating tumor DNA
Xing Lu1, Liqian Wang2, Qingxue Chen1
1College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, China. lei.li@mail.zjxu.edu.cn gbzhou@mail.zjxu.edu.cn.
Chemical Communications (Cambridge, England)
|March 14, 2020
Summary
A novel artificial multifunctional allostery (mFA) strategy enables DNA nanowire assembly for a sensitive biosensor. This biosensor quantifies circulating tumor DNA (ctDNA) in serum with tunable detection capabilities.
Area of Science:
- Biotechnology
- Nanotechnology
- Molecular Biology
Background:
- Circulating tumor DNA (ctDNA) is a crucial biomarker for cancer detection and monitoring.
- Developing sensitive and selective biosensors for ctDNA analysis remains a significant challenge.
Purpose of the Study:
- To develop a novel strategy for regulating DNA nanowire assembly using artificial multifunctional allostery (mFA).
- To engineer a highly sensitive and selective biosensor for the quantitative analysis of ctDNA in serum samples.
Main Methods:
- Utilized artificial multifunctional allostery (mFA) to control DNA nanowire formation initiated by ctDNA.
- Engineered a single-step, amplified, and dynamic biosensor based on the mFA-regulated DNA nanowire.
Main Results:
- Successfully demonstrated the regulation of DNA nanowire assembly using the mFA strategy.
- The developed biosensor achieved quantitative analysis of ctDNA in serum with tunable sensitivity and selectivity.
Conclusions:
- The mFA strategy provides a versatile platform for constructing advanced DNA nanostructures.
- The engineered biosensor shows great potential for non-invasive cancer diagnostics through ctDNA detection.
