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Updated: Dec 25, 2025

Standardized In vitro Assays to Visualize and Quantify Interactions between Human Neutrophils and Staphylococcus aureus Biofilms
Published on: June 8, 2022
Conditioning adhesive contacts between the neutrophils and the endotheliocytes by Staphylococcus aureus
Svetlana N Pleskova1,2, Ruslan N Kriukov1, Sergey Z Bobyk1
1Research and Education Center for Physics of Solid State Nanostructures, Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia.
Abstract:
We have developed a model for evaluating the integral intercellular interactions in the "endotheliocyte-neutrophil" system and have shown the high variability of adhesion contacts in different donors associated with different expression profiles of neutrophils. Two methods (forсe spectroscopy-spectroscopy and scanning ion-conductance microscopy) showed a decrease in the rigidity of the membrane-cytoskeletal complex of neutrophils under the influence of Staphylococcus aureus 2879 M. Adding this strain to the "endotheliocyte-neutrophil" system caused a statistically significant decrease in the adhesion force and adhesion work, which indicates a change in the expression profile and physicochemical properties of membranes of both types of interacting cells (neutrophils and endotheliocytes).
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