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Updated: Dec 24, 2025

Lensless Fluorescent Microscopy on a Chip
Published on: August 17, 2011
Single-shot lensless imaging with fresnel zone aperture and incoherent illumination
Jiachen Wu1, Hua Zhang1, Wenhui Zhang1
11State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, 100084 Beijing, China.
Abstract:
Lensless imaging eliminates the need for geometric isomorphism between a scene and an image while allowing the construction of compact, lightweight imaging systems. However, a challenging inverse problem remains due to the low reconstructed signal-to-noise ratio. Current implementations require multiple masks or multiple shots to denoise the reconstruction. We propose single-shot lensless imaging with a Fresnel zone aperture and incoherent illumination. By using the Fresnel zone aperture to encode the incoherent rays in wavefront-like form, the captured pattern has the same form as the inline hologram. Since conventional backpropagation reconstruction is troubled by the twin-image problem, we show that the compressive sensing algorithm is effective in removing this twin-image artifact due to the sparsity in natural scenes. The reconstruction with a significantly improved signal-to-noise ratio from a single-shot image promotes a camera architecture that is flat and reliable in its structure and free of the need for strict calibration.
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