Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

9.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.6K
Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

5.7K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
5.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Absence of GDF15 Aggravates Pressure Overload-Induced Cardiac Remodelling in Mice Hallmarked by Perivascular Fibrosis and Signs of Endothelial-to-Mesenchymal Transition.

International journal of molecular sciences·2026
Same author

Extracellular Vesicles Released by Picornavirus-Infected Cells Modify Antiviral Immune Cell Responses.

Journal of extracellular vesicles·2026
Same author

Efficiency and immunogenicity of lipid nanoparticle-mediated cardiac mRNA delivery are lipid composition-dependent.

Molecular therapy. Nucleic acids·2026
Same author

Author Correction: Engineering anti-BCMA CAR T cells for enhancing myeloma killing efficacy via apoptosis regulation.

Nature communications·2026
Same author

EV-Mediated Intracardiac Crosstalk Mitigates Doxorubicin-Induced Cardiotoxicity.

Circulation research·2026
Same author

Photoporation enables non-viral delivery of prime editing RNP complexes into human iPSC-derived cardiomyocytes for cardiac genome correction.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026

Related Experiment Video

Updated: Dec 24, 2025

Isolation, Transfection, and Long-Term Culture of Adult Mouse and Rat Cardiomyocytes
09:17

Isolation, Transfection, and Long-Term Culture of Adult Mouse and Rat Cardiomyocytes

Published on: October 10, 2020

9.9K

Non-coding RNAs in Cardiac Regeneration.

C F T van der Ven1, B C R Hogewoning1, A van Mil1

  • 1Laboratory of Experimental Cardiology, UMC Utrecht Regenerative Medicine Center, University Utrecht, University Medical Center Utrecht, Utrecht, The Netherlands.

Advances in Experimental Medicine and Biology
|April 15, 2020
PubMed
Summary

Heart failure is a growing global crisis. Current stem cell therapies show limited success, shifting focus to reactivating the heart's own cardiomyocyte regeneration for improved cardiac repair.

More Related Videos

Myocardial Infarction in Neonatal Mice, A Model of Cardiac Regeneration
07:48

Myocardial Infarction in Neonatal Mice, A Model of Cardiac Regeneration

Published on: May 24, 2016

19.5K
In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter
05:04

In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter

Published on: February 22, 2022

3.7K

Related Experiment Videos

Last Updated: Dec 24, 2025

Isolation, Transfection, and Long-Term Culture of Adult Mouse and Rat Cardiomyocytes
09:17

Isolation, Transfection, and Long-Term Culture of Adult Mouse and Rat Cardiomyocytes

Published on: October 10, 2020

9.9K
Myocardial Infarction in Neonatal Mice, A Model of Cardiac Regeneration
07:48

Myocardial Infarction in Neonatal Mice, A Model of Cardiac Regeneration

Published on: May 24, 2016

19.5K
In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter
05:04

In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter

Published on: February 22, 2022

3.7K

Area of Science:

  • Cardiovascular Research
  • Regenerative Medicine
  • Stem Cell Biology

Background:

  • Cardiovascular disease is a leading cause of death, with heart failure incidence rising.
  • Limited donor hearts for transplantation necessitate alternative treatments for end-stage heart failure.
  • Existing cardiomyocyte regenerative capacity is insufficient for functional myocardial repair after injury.

Purpose of the Study:

  • To review the evolution of stem cell-based strategies for cardiac regeneration.
  • To evaluate the efficacy and limitations of cardiac progenitor cells (CPCs) in myocardial repair.
  • To explore the shift towards endogenous cardiomyocyte proliferation as a therapeutic approach.

Main Methods:

  • Systematic review of pre-clinical studies on cardiac stem cell therapies in animal models.
  • Analysis of evidence regarding hematopoietic stem cells and CPCs differentiation potential.
  • Examination of recent single-cell sequencing and lineage tracing studies in murine hearts.

Main Results:

  • Early studies suggested hematopoietic stem cells could regenerate myocardium, but later evidence indicated differentiation into hematopoietic lineages only.
  • Cardiac progenitor cells (CPCs) showed minimal contribution to functional cardiomyocyte generation despite positive functional outcomes in some studies.
  • Recent research challenges the existence of a quiescent cardiac stem cell population and supports endogenous cardiomyocyte proliferation via dedifferentiation and cell cycle re-entry.

Conclusions:

  • Stem cell therapies, including CPCs, have shown limited success in directly generating functional cardiomyocytes.
  • The focus in cardiac regeneration research is shifting towards harnessing the intrinsic regenerative potential of existing cardiomyocytes.
  • Future strategies may involve manipulating cardiomyocyte cell-cycle activity, reprogramming, and utilizing small molecules or RNA regulation for cardiac repair.