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Ano6 disruption impairs acinar cell regulatory volume decrease and protein secretion in murine submandibular salivary
Takashi Munemasa1,2, Xin Gao1, James E Melvin1
1Secretory Mechanisms and Dysfunctions Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland.
Journal of Cellular Physiology
|April 25, 2020
Summary
Anoctamin 6 (Ano6) is crucial for salivary gland cell volume regulation and protein secretion, particularly in females. Its absence significantly impairs regulatory volume decrease (RVD) and affects β-adrenergic-dependent protein release.
Area of Science:
- Cell biology
- Physiology
- Ion channel function
Background:
- Anoctamin 6 (Ano6) is a widely expressed calcium-dependent protein.
- Its specific functions in salivary glands remain largely uncharacterized.
- Ano6 is implicated in various cellular processes.
Purpose of the Study:
- To investigate the role of Ano6 in salivary gland acinar cell function.
- To determine Ano6's involvement in regulatory volume decrease (RVD) and secretion.
- To explore potential sex-specific effects of Ano6 in salivary glands.
Main Methods:
- Utilized Ano6 knockout (Ano6-/-) mouse models.
- Assessed regulatory volume decrease (RVD) in submandibular gland (SMG) acinar cells.
- Measured ex vivo agonist-stimulated secretion of water, ions, and proteins.
Main Results:
- Ano6 deficiency significantly reduced RVD by approximately 70% in SMG acinar cells.
- Ano6 is essential for the calcium-dependent RVD pathway in salivary glands.
- Ano6 disruption did not affect water and ion secretion but reduced female-specific β-adrenergic-dependent protein secretion.
Conclusions:
- Ano6 plays a significant role in regulating salivary gland acinar cell volume.
- Ano6 modulates β-adrenergic-stimulated protein secretion, with a notable effect in female SMGs.
- Ano6's function extends to both cell volume homeostasis and specific secretory pathways in salivary glands.
