Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Integrative profiling of glymphatic dysfunction in adolescent subthreshold depression.

Journal of affective disorders·2026

Same author

Recent advances in nanomaterial-based strategies for chronic pain alleviation.

Materials today. Bio·2026

Same author

Oral JAK inhibitors combined with 308-nm excimer laser in pediatric progressive vitiligo: Further considerations and future directions.

Journal of the American Academy of Dermatology·2026

Same author

A synergistic nanozyme platform breaking the neuroinflammatory-oxidative stress cycle for extended pain relief.

International journal of pharmaceutics: X·2026

Same author

A set-theoretic configurational analysis of sports event policy change: insights from Shanghai.

Frontiers in physiology·2026

Same author

Green Interfacial Engineering via Carbon Dot Grafting for Strong and Aging-Resistant Fiber-Elastomer Interfaces.

ACS applied materials & interfaces·2026

Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026

Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026

Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026

Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026

Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026

Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 21, 2025

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Published on: January 30, 2020

Isotropic three-dimensional imaging with lattice light-sheet difference microscopy.

Chengfeng Zhang, Yuchen Chen, Wenjie Liu

|May 16, 2020

Summary

This summary is machine-generated.

Lattice light-sheet microscopy (LLSM) can now achieve nearly isotropic spatial resolution with reduced sidelobes using a novel optical lattice. This advancement, termed lattice light-sheet difference microscopy (LLSDM), enhances imaging capabilities for life sciences.

More Related Videos

Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy

Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy

Published on: May 22, 2020

$On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature$

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature

Published on: March 11, 2022

Related Experiment Videos

Last Updated: Dec 21, 2025

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Published on: January 30, 2020

Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy

Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy

Published on: May 22, 2020

$On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature$

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature

Published on: March 11, 2022

Area of Science:

Optical microscopy
Biophotonics
Superresolution microscopy

Background:

Lattice light-sheet microscopy (LLSM) provides fast imaging but struggles with axial resolution.
Structured illumination microscopy (SIM) in LLSM offers high but anisotropic resolution at reduced speed.

Purpose of the Study:

To introduce a novel optical lattice for lattice light-sheet microscopy.
To improve spatial resolution and reduce artifacts in light-sheet imaging.

Main Methods:

Development of a new optical lattice using composed beam vectors with different phases.
Implementation of lattice light-sheet difference microscopy (LLSDM) by switching between conventional and new optical lattices.
Feasibility examination through simulations using real acquisition parameters and microscope properties.

Main Results:

Achieved nearly isotropic spatial resolution.
Significantly alleviated sidelobe artifacts.
Demonstrated feasibility of LLSDM through simulations.

Conclusions:

LLSDM offers enhanced resolution and artifact reduction compared to conventional LLSM.
The proposed optical lattice broadens the imaging applications of light-sheet microscopes.
LLSDM facilitates advanced applications in life sciences research.