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Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution
Published on: May 2, 2020
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Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution
Nan Chen1, Kai Zhu2, Shiqiang Yan3
1Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University; Shanghai Institute of Cardiovascular Diseases.
Journal of Visualized Experiments : Jove
|May 19, 2020
Summary
This study introduces a multilayered bioink strategy to prevent cell sedimentation in 3D bioprinting. This method enhances cell distribution and improves the application of low-viscosity bioinks for better cell survival.
Area of Science:
- Biotechnology
- Materials Science
- Cell Biology
Background:
- Extrusion-based 3D bioprinting utilizes low-viscosity bioinks for cell protection.
- Cellular sedimentation in liquid bioinks causes inhomogeneous distribution, limiting applications.
Purpose of the Study:
- To develop a novel strategy to prevent cell sedimentation in liquid-like bioinks.
- To improve cell distribution and viability in 3D bioprinted constructs.
Main Methods:
- A multilayered modified strategy was developed for liquid-like bioinks.
- Multiple liquid interfaces were engineered to create interfacial retention.
- The strategy was tested using gelatin methacryloyl bioinks.
Main Results:
- The multilayered system effectively retarded cell sedimentation.
- Interfacial retention surpassed the gravitational pull on cells.
- A more homogeneous cell dispersion was achieved in the bioink.
Conclusions:
- The multilayered modified strategy successfully prevents cell sedimentation in low-viscosity bioinks.
- Interfacial retention is key to maintaining homogeneous cell distribution.
- This approach enhances the utility of liquid-like bioinks in 3D bioprinting.
