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Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

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An ultraportable and versatile point-of-care DNA testing platform.

Huan Xu¹, Anyue Xia², Dandan Wang³

¹Department of Clinical Laboratory Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.

Science Advances

|May 20, 2020

Summary

A new ultraportable point-of-care testing (POCT) kit, POCKET, analyzes DNA from various sources. This versatile platform offers sensitive, rapid, and stable detection for diverse applications.

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Area of Science:

Biotechnology
Analytical Chemistry
Diagnostics

Background:

Point-of-care testing (POCT) is crucial in resource-limited settings.
Existing POCT platforms often lack versatility and portability.

Purpose of the Study:

To demonstrate an ultraportable and versatile POCT platform, POCKET, for sample-to-answer DNA analysis.
To showcase POCKET's applicability across clinical, environmental, food, and agricultural fields.

Main Methods:

Developed POCKET: an ultraportable (<100 g, <25 cm) kit with an integrated chip (i-chip) and foldable box (f-box).
The i-chip features integrated sample preparation and a novel triple signal amplification system.
The f-box utilizes a smartphone for heating, signal detection, and result readout.

Main Results:

POCKET achieved sensitive DNA detection (<10^3 copies/ml) with single-base specificity.
The platform demonstrated rapid results (<2 hours) and a shelf life of over 10 weeks.
Successfully detected various DNA types in clinical, environmental, food, and agricultural samples.

Conclusions:

The inexpensive, ultraportable POCKET platform offers a versatile sample-to-answer solution.
POCKET has significant potential for clinical diagnostics, food safety, agricultural protection, and environmental monitoring.