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Bioinformatics Analysis based on Multiple Databases Identifies Hub Genes Associated with Hepatocellular Carcinoma.
Lu Zeng1,2, Xiude Fan1, Xiaoyun Wang1
1Department of Infectious Diseases, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, P.R. China.
Current Genomics
|June 2, 2020
Summary
Nine hub genes, including TOP2A and CDK1, were identified as potential biomarkers for hepatocellular carcinoma (HCC). These genes are associated with HCC prognosis and may serve as therapeutic targets for liver cancer.
Area of Science:
- Oncology
- Genetics
- Bioinformatics
Background:
- Hepatocellular carcinoma (HCC) is the most prevalent form of liver cancer.
- The underlying mechanisms driving hepatocarcinogenesis are not fully understood.
Purpose of the Study:
- To identify key genes (hub genes) associated with hepatocellular carcinoma (HCC) by analyzing data from multiple public databases.
- To explore the potential of these hub genes as diagnostic or therapeutic targets for HCC.
Main Methods:
- Utilized Gene Expression Omnibus (GEO) datasets (GSE45267, GSE60502, GSE74656) to identify differentially expressed genes (DEGs) in HCC.
- Performed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses on aggregated DEGs.
- Constructed a protein-protein interaction (PPI) network to identify hub genes and analyzed their association with HCC patient prognosis using The Cancer Genome Atlas (TCGA) data.
Main Results:
- Identified a total of 221 aggregated DEGs across the datasets, significantly enriched in cell cycle and metabolic pathways.
- Nine hub genes (TOP2A, NDC80, CDK1, CCNB1, KIF11, BUB1, CCNB2, CCNA2, TTK) were pinpointed through PPI network analysis.
- All nine identified hub genes demonstrated a significant association with the prognosis of hepatocellular carcinoma patients.
Conclusions:
- TOP2A, NDC80, CDK1, CCNB1, KIF11, BUB1, CCNB2, CCNA2, and TTK are identified as crucial hub genes in hepatocellular carcinoma (HCC).
- These hub genes represent potential biomarkers for HCC diagnosis and prognosis.
- The identified hub genes may serve as promising therapeutic targets for the treatment of HCC.
