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Circulating tumor necrosis factor-α DNA are elevated in psoriasis.
Ryoko Sakamoto1, Soichiro Sawamura1, Ikko Kajihara1
1Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.
The Journal of Dermatology
|June 10, 2020
Summary
Circulating tumor necrosis factor-alpha (TNF-α) DNA levels in cell-free DNA (cfDNA) are significantly elevated in psoriasis patients, serving as a potential diagnostic and severity biomarker for this inflammatory skin condition.
Area of Science:
- Dermatology
- Molecular Diagnostics
- Immunology
Background:
- Tumor necrosis factor-alpha (TNF-α) is a key pro-inflammatory cytokine in psoriasis pathogenesis.
- Elevated serum TNF-α levels are recognized biomarkers for psoriasis.
- Circulating cell-free DNA (cfDNA) reflects cellular damage and is emerging as a diagnostic marker across various diseases.
Purpose of the Study:
- To investigate the presence and levels of TNF-α DNA within cfDNA.
- To evaluate the potential of circulating TNF-α DNA as a diagnostic biomarker for psoriasis.
- To determine if TNF-α DNA levels correlate with psoriasis severity.
Main Methods:
- Serum cfDNA was isolated from 79 patients with psoriasis vulgaris and 29 with psoriatic arthritis.
- Droplet digital polymerase chain reaction (ddPCR) was employed to quantify TNF-α DNA levels.
- Correlation analysis was performed between TNF-α DNA levels and the Psoriasis Area and Severity Index (PASI) score.
Main Results:
- Circulating TNF-α DNA levels were significantly higher in psoriasis patients compared to healthy controls (AUC = 0.91).
- Positive correlation observed between TNF-α DNA copy levels and PASI scores in patients with severe psoriasis (PASI > 10).
Conclusions:
- Serum TNF-α DNA levels show promise as a diagnostic biomarker for psoriasis.
- Circulating TNF-α DNA may serve as a biomarker for assessing disease severity in patients with severe psoriasis.
- Further research is warranted to establish serum TNF-α DNA as a novel psoriasis biomarker.
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