Touchscreen-based assessment of risky-choice in mice

Addictions are characterized by choices made to satisfy the addiction despite the risk it could produce an adverse consequence. Here, we developed a murine version of a 'risky decision-making' task (RDT), in which mice could respond on a touchscreen panel to obtain either a large milkshake reward associated with varying probability of footshock, or a smaller amount of the same reward that was never punished. Results showed that (the following font is incorrectly smaller/subscripted) mice shifted choice from the large to small reward stimulus as shock probability increased. Immunohistochemical analysis revealed more Fos-positive cells in prelimbic cortex (PL) and basal amygdala (BA) after RDT testing, and a strong anti-correlation between infralimbic cortex (IL) activity and choice of the large reward stimulus under likely (75-100 % probability) punishment. These findings establish an assay for risky choice in mice and provide preliminary insight into the underlying neural substrates.