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Hydroxyapatite Surfaces Functionalized with a Self-Assembling Peptide: XPS, RAIRS and NEXAFS Study.
Valeria Secchi1,2, Stefano Franchi1,3, Monica Dettin4
1Department of Science, Roma Tre University, Via della Vasca Navale 79, 00146 Rome, Italy.
Nanomaterials (Basel, Switzerland)
|June 18, 2020
Summary
Hydroxyapatite coatings on titanium alloys were functionalized with self-assembling peptides (SAPs) to enhance bioactivity for orthopedic prostheses. The study confirmed SAP adsorption and ordered structures, with layer thickness dependent on coating composition.
Area of Science:
- Biomaterials Science
- Surface Chemistry
- Orthopedic Engineering
Background:
- Titanium alloys like Ti6Al4V are crucial for orthopedic prostheses.
- Hydroxyapatite (HAP) coatings enhance biocompatibility and bioactivity of metallic implants.
- Functionalizing HAP with self-assembling peptides (SAPs) aims to further improve osseointegration.
Purpose of the Study:
- To investigate the adsorption and structural characteristics of self-assembling peptides (SAPs) on modified hydroxyapatite (HAP) coatings.
- To evaluate the influence of HAP substrate composition (Mg, Si, Ti enrichment) and solution pH on SAP adsorption and stability.
- To assess the potential of SAP-functionalized HAP coatings for enhanced orthopedic implant bioactivity.
Main Methods:
- Deposition of HAP coatings enriched with Mg and Si or Ti onto Ti6Al4V substrates using RF magnetron sputtering.
- Functionalization of HAP coatings via incubation in a specific self-assembling peptide (SAP) solution.
- Surface analysis using X-Ray Photoelectron Spectroscopy (XPS), Fourier Transform Infrared (FTIR), and Near Edge X-Ray Absorption Fine Structure (NEXAFS) spectroscopies.
Main Results:
- Experimental evidence confirmed the adsorption of SAPs onto the HAP-coated surfaces.
- The adsorbed SAP overlayers exhibited ordered structures with defined molecular orientation.
- The thickness of the SAP overlayer was found to be dependent on the specific composition of the underlying HAP coating.
- Peptide stability and structure were influenced by the HAP substrate and the pH of the SAP solution.
Conclusions:
- Self-assembling peptides (SAPs) can be successfully adsorbed onto modified hydroxyapatite (HAP) coatings, forming ordered overlayers.
- The composition of HAP coatings significantly influences the SAP overlayer thickness and structure.
- SAP-functionalized HAP coatings show promise for improving the bioactivity of orthopedic implants, warranting further investigation into their in vivo performance.
