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Updated: Dec 15, 2025

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
Published on: April 8, 2017
An in vitro site-specific cleavage assay of CRISPR-Cas9 using a personal glucose meter
Shaohua Gong1, Yuanyuan Chen1, Wei Pan1
1College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan 250014, P. R. China. panwei@sdnu.edu.cn lina@sdnu.edu.cn tangb@sdnu.edu.cn.
Abstract:
A sensitive and cost-effective assay based on a personal glucose meter was developed to detect the in vitro site-specific cleavage efficiency of CRISPR-Cas9.

