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Bioluminescent microassay of various metabolites using bacterial luciferase co-immobilized with multienzyme systems
N N Ugarova1, O V Lebedeva, I G Frumkina
1Chemistry Department, Lomonosov Moscow State University, USSR.
Analytical Biochemistry
|September 1, 1988
Abstract:
Co-immobilization methods have been developed for a bienzymatic system of luminescent Beneckea harveyi bacteria with formate dehydrogenase, glucose-6-phosphate dehydrogenase, and phosphoglucomutase. Bioluminescent assays have been devised for NADH, NAD, FMN, glucose 6-phosphate, and glucose 1-phosphate using the co-immobilized enzyme preparation. The lowest detection limits were in the picomole range with the bacterial extract and in the femtomole range with the partially purified enzymes, bacterial luciferase, and NADH:FMN oxidoreductase.