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Updated: Dec 13, 2025

Author Spotlight: The Production of Recombinant Proteins
Published on: June 30, 2023
Production of Recombinant Protein Self-Assembly Nanocontainers in a Prokaryotic System
L A Ovchinnikova1, S S Terekhov1, I N Filimonova1
1M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Abstract:
We optimized a method for the preparation and purification of self-assembled protein nanocontainers EPN, the latest achievement in protein engineering. These nanocontainers are highly stable and provide the possibility of highly specific loading of a protein therapeutic drug. The described technique can be proposed as a tool for production of nanocontainers in a prokaryotic system. The obtained nanocontainers for the delivery of protein preparations can be used in the treatment of chronic, autoimmune, and oncological diseases.

