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Updated: Dec 12, 2025
Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor
Published on: August 1, 2022
Decellularization systems and devices: State-of-the-art
Deepak Choudhury1, Marcus Yee2, Zach Lee Jia Sheng1
1Biomanufacturing Technology Group, Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A*STAR), 138668, Singapore; Singapore Institute of Manufacturing Technology (SIMTech), Agency for Science, Technology, and Research (A*STAR), 2 Fusionopolis Way, #08-04, Innovis 138634, Singapore.
Decellularization removes cells from tissues to create extracellular matrix (ECM) scaffolds for tissue engineering. Current technologies are lab-scale and non-standardized, hindering commercialization of decellularized ECM (dECM).
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Extracellular matrix (ECM) provides structural and biochemical support for cells, forming tissues and organs.
- Decellularized ECM (dECM) is derived from natural tissues, serving as a versatile biomaterial for tissue regeneration.
- dECM is utilized in bioinks and as scaffolds for recellularization, aiming to rebuild functional organs.
Purpose of the Study:
- To review current decellularization systems and devices for producing decellularized ECM (dECM).
- To discuss the challenges and limitations of existing decellularization technologies.
- To suggest pathways for scaling up and commercializing dECM for medical applications.
Main Methods:
- Review of existing literature on decellularization systems and devices.
- Categorization of decellularization technologies used in academic and commercial settings.
- Analysis of the standardization and scalability of current dECM production methods.
Main Results:
- Decellularization technologies are largely non-standardized and limited to small, lab-specific scales.
- A lack of standardized protocols exists for assessing dECM quality and consistency.
- Current systems present challenges for large-scale production and commercialization.
Conclusions:
- Standardization of decellularization processes and devices is crucial for reproducible dECM production.
- Further technological development is needed to enable large-scale manufacturing of dECM.
- Commercialization of dECM requires addressing scalability, standardization, and quality control.
