Topical Corneal Cross-Linking Solution Delivered Via Corneal Reservoir in Dutch-Belted Rabbits.

Area of Science:

• Ophthalmology
• Corneal Science
• Biomaterials

Background:

• Standard photochemical cross-linking (UV-riboflavin) for corneal stabilization has limitations.
• Developing topical corneal cross-linking solutions is a significant area of research.

Purpose of the Study:

• To develop optimal concentration and delivery methods for topical corneal stabilization in living rabbit eyes.
• To evaluate the safety and efficacy of a novel topical corneal cross-linking solution.

Main Methods:

• Dutch-belted rabbits were treated with topical sodium hydroxymethylglycinate solutions (10-250 mM) via corneal reservoir.
• Application regimens included a one-time 30-minute application or weekly 5-minute applications for 7 weeks.
• Evaluations included intraocular pressure, pachymetry, epithelial/endothelial cell counts, keratocyte changes, and post-mortem mechanical inflation testing.

Main Results:

• Topical cross-linking showed minimal impact on intraocular pressure, pachymetry, and endothelial cells.
• A 30-minute application caused transient corneal thickening and temporary epithelial damage.
• A 20 mM concentration applied for 30 minutes demonstrated the most significant corneal stiffening effect.

Conclusions:

• Safe and effective topical corneal cross-linking conditions were identified in living rabbit eyes.
• The developed method increases corneal stiffness and strength.
• This approach holds potential for treating conditions like keratoconus.