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Updated: Dec 9, 2025

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
Published on: July 25, 2019
Function and regulation of the Spt-Ada-Gcn5-Acetyltransferase (SAGA) deubiquitinase module
Dayanne V Cornelio-Parra1, Rituparna Goswami1, Kara Costanzo1
1University of Missouri, Kansas City, MO, USA.
Abstract:
The Spt-Ada-Gcn5 Acetyltransferase (SAGA) chromatin modifying complex is a critical regulator of gene expression and is highly conserved across species. Subunits of SAGA arrange into discrete modules with lysine aceyltransferase and deubiquitinase activities housed separately. Mutation of the SAGA deubiquitinase module can lead to substantial biological misfunction and diseases such as cancer, neurodegeneration, and blindness. Here, we review the structure and functions of the SAGA deubiquitinase module and regulatory mechanisms acting to control these.
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