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Related Experiment Video

Updated: Dec 9, 2025

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

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Published on: January 3, 2017

Hydrogel Network Dynamics Regulate Vascular Morphogenesis.

Zhao Wei¹, Rahel Schnellmann¹, Hawley C Pruitt¹

¹Department of Chemical and Biomolecular Engineering, The Institute for NanoBioTechnology, Physical Sciences-Oncology Center, Johns Hopkins University, Baltimore, MD 21218, USA.

|September 15, 2020

Summary

This summary is machine-generated.

Dynamic hydrogels promote cell contractility and vascular tissue formation by activating integrin signaling. This research highlights the crucial role of matrix dynamics in vascular morphogenesis and tissue engineering.

Keywords:

cell contractility integrin clustering stress-relaxation vasculogenesis

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Area of Science:

Biomaterials Science
Cell Biology
Tissue Engineering

Background:

Matrix dynamics significantly influence multicellular tissue development.
Understanding vascular morphogenesis requires investigating cell-matrix interactions.

Purpose of the Study:

To investigate mechanisms of vascular morphogenesis using hydrogels with varying crosslinking capacities.
To elucidate the role of matrix dynamics in endothelial cell behavior and tissue formation.

Main Methods:

Development of dynamic (D) and non-dynamic (N) hydrogels with identical polymer components.
Assessment of human endothelial colony-forming cells (hECFCs) contractility, integrin clustering, and focal adhesion kinase (FAK) signaling.
In vitro and in vivo evaluation of vascular assembly and angiogenesis.

Main Results:

Dynamic hydrogels enhanced hECFC contractility, integrin β1 clustering, and FAK signaling, leading to robust vasculature assembly.
Non-dynamic hydrogels and stiff hydrogels (both D and N) did not promote FAK signaling or vascular morphogenesis.
Dynamic hydrogels promoted microvessel formation and angiogenesis in vivo.

Conclusions:

Cell contractility mediates integrin signaling through inside-out signaling pathways.
Matrix dynamics are critical for vascular tissue formation, impacting cell signaling and morphogenesis.
Findings inform future vascularization and tissue engineering strategies.