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Updated: Dec 8, 2025
Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats
Published on: October 13, 2018
; PUBERTY GENESIS OF FEMALES-OFFSPRING RATS BORN TO MOTHERS WITH FETOPLACENTAL INSUFFICIENCY
N Seliukova1, M Boyko2, S Kustova2
11SI «V. Danilevsky Institute for Endocrine Pathology Problems of NAMS of Ukraine»; 2National University of Pharmacy, Kharkiv, Ukraine.
Insights
Fetoplacental insufficiency (FPI) negatively impacts female offspring puberty development, especially in older mothers. Drug therapy during pregnancy showed promise in normalizing reproductive system development in offspring exposed to FPI.
Area of Science:
- Reproductive biology
- Developmental toxicology
- Endocrinology
Background:
- Fetoplacental insufficiency (FPI) can disrupt fetal development.
- Maternal age may influence the impact of FPI on offspring development.
Purpose of the Study:
- Investigate puberty development in female offspring born to mothers with FPI across different reproductive ages.
- Evaluate the efficacy of pharmaceutical interventions during pregnancy for FPI-related developmental issues.
Main Methods:
- Comparative analysis of puberty markers in female offspring from mothers with and without FPI.
- Histological examination of ovarian structure.
- Assessment of drug therapy efficacy.
Main Results:
- FPI negatively affects puberty genesis, with distinct effects based on maternal age.
- Reproductively young mothers' offspring showed estrogen deficiency signs (increased anogenital distance).
- Reproductively mature mothers' offspring exhibited accelerated sexual development, increased body mass, and ovarian disturbances (follicle density, ratio, atresia).
Conclusions:
- FPI poses significant risks to female offspring reproductive system development.
- Maternal age modulates the severity and type of developmental abnormalities.
- The tested pharmaceutical composition demonstrated superior normalization effects compared to the control drug.
Abstract:
The purpose of this scientific work was to investigate the development of puberty in females-offspring born to mothers of different age with fetoplacental insufficiency (FPI) and to evaluate efficacy of base and combined drug therapy during pregnancy. Negative influence of FPI on the puberty genesis of females-offspring born to mothers of different reproductive age is considered to be the results of the investigation. In particular, the increased anogenital distance, which is the sign of estrogen deficiency, has been observed in females-offspring born to reproductively young mothers with FPI. Females-offspring born to reproductively matured mothers with FPI have demonstrated more negative changes of reproductive system development. That is, body mass and anogenital distance increasing amid accelerated sexual development have been detected. The increasing of testosterone level has caused inadequate ovaries stimulation which has led to steroid genesis disturbances. During histological investigation of ovarian structure of pubertal rats born to mothers of both groups of age, the decreasing of follicles density, the disturbance in follicles types ratio - early secondary follicles were prevailed, declining folliculogenesis reserve and increased number of atretical follicles have been observed. The introduction of pharmaceutical composition to pregnant rats of both groups of age amid fetoplacental insufficiency leads to stronger normalization of reproductive system development in females-offspring than using of drug of comparison.
