Related Experiment Video
Updated: Dec 7, 2025

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
Published on: June 24, 2013
Microscopic Structure from Motion (SfM) for Microscale 3D Surface Reconstruction
1Mechanical Engineering, Texas A&M-Corpus Christi, Corpus Christi, TX 78412, USA.
Abstract:
In microscale photogrammetry, the confocal microscopic imaging technique has been the dominant trend. Unlike the confocal imaging mostly for transparent objects, we propose a novel method to construct a 3D shape in microscale for various micro-sized solid objects in a broad spectrµm of applications. Recently, the structure from motion (SfM) demonstrated reliable 3D reconstruction capability for macroscale objects. In this paper, we discuss the results of a novel micro-surface reconstruction method using the Structure from Motion in microscale. The proposed micro SfM technique utilizes the photometric stereovision via microscopic photogrammetry. The main challenges lie in the scanning methodology, ambient light control, and light conditioning for microscale object photography. Experimental results of the microscale SfM, as well as the modeling accuracy analysis of a reconstructed micro-object, are shared in the paper.
More Related Videos
06:33Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
Published on: October 29, 2019
14:23Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
Published on: March 6, 2018
Related Concept Videos
Electron Microscope Tomography and Single-particle Reconstruction
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Three-Dimensional Microscopy in Microbiology