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Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate
Published on: December 12, 2015
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Performance of Predictive Equations and Biochemical Measures Quantifying Net Endogenous Acid Production and the
Benjamin H Parmenter1,2, Michael Dymock3, Tanushree Banerjee4
1School of Biomedical Sciences, University of Western Australia, Royal Perth Hospital, Perth, Western Australia, Australia.
Kidney International Reports
|October 26, 2020
Summary
This study evaluated methods for determining net endogenous acid production (NEAP) and diet-dependent acid-base load. Some diet equations accurately estimated NEAP, but all methods have limitations for human health research.
Area of Science:
- Biochemistry
- Human Nutrition
- Metabolic Balance Studies
Background:
- Limited studies assess accuracy of net endogenous acid production (NEAP) methods.
- Investigated performance of methods quantifying diet-dependent acid-base load.
Purpose of the Study:
- To compare biochemical measures and diet equations for NEAP and acid-base load.
- To assess accuracy and precision of different estimation methods.
Main Methods:
- Used metabolic balance study data to calculate NEAP via biochemical measures (NAE, U_NEAP, U_PRAL) and diet equations.
- Compared methods in healthy participants on acid-forming and base-forming diets.
Main Results:
- Bland-Altman analysis showed good accuracy but modest precision for U_NEAP vs. NAE.
- Diet equations by Sebastian et al. (PRAL), Lemann et al. (NEAP), and Remer and Manz (NEAP) showed accurate bias.
Conclusions:
- Encourages collection of U_PRAL and U_NEAP measures.
- Highlights limitations of all methods when linking diet-acid load to human health.
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