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Updated: Dec 3, 2025

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells
Published on: October 1, 2010
Heterologous Expression of Ion Channels in Mammalian Cell Lines
Alistair Mathie1, Emma L Veale2, Robyn G Holden2
1Medway School of Pharmacy, University of Kent and University of Greenwich, Chatham Maritime, UK. a.a.mathie@kent.ac.uk.
Abstract:
Heterologous expression of recombinant ion channel subunits in mammalian cell lines allows for characterization of their functional properties and pharmacological regulation. In this chapter, we describe methods for thawing, refreezing, passaging, cell culture, and transfection of tsA201 cells suitable for electrophysiology and imaging experiments. Furthermore, we discuss the strengths and limitations of using these methods.
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