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GmLCLs negatively regulate ABA perception and signalling genes in soybean leaf dehydration response
Li Yuan1, Grace Z Xie2, Siyuan Zhang3
1Key Laboratory of Molecular and Cell Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang, China.
Plant, Cell & Environment
|October 30, 2020
Summary
Soybean circadian clock genes (GmLCLs) regulate plant responses to drought. The quadruple mutant showed reduced water loss, indicating GmLCLs are negative regulators of abscisic acid (ABA) signaling during dehydration.
Area of Science:
- Plant biology
- Circadian rhythms
- Drought stress response
Background:
- Plants use circadian clocks to adapt to daily environmental changes.
- Circadian oscillators influence abscisic acid (ABA) signaling under drought, but mechanisms are unclear in crops.
- Soybean circadian clock genes (GmLCLs) role in water stress is investigated.
Purpose of the Study:
- Investigate the function of soybean circadian clock genes (GmLCLa1, GmLCLa2, GmLCLb1, GmLCLb2) in response to leaf water stress.
- Elucidate the molecular mechanisms by which circadian oscillators gate ABA signaling during dehydration in soybean.
Main Methods:
- Analysis of a quadruple mutant (gmlclq) of soybean circadian clock genes under dehydration stress.
- Gene expression profiling of GmLCLs and ABA signaling-related genes.
- Measurement of leaf water loss.
Main Results:
- The gmlclq quadruple mutant exhibited decreased leaf water loss under dehydration stress.
- Dehydration delayed peak expression of GmLCL genes by 4 hours.
- In the gmlclq mutant, diurnal expression of ABA receptor, catabolism, and signaling genes shifted to daytime, with increased expression of GmPYL17, GmCYP707A, GmABI2, and GmSnRK2s under stress.
Conclusions:
- Soybean GmLCLs function as negative regulators of ABA signaling in leaves during dehydration.
- Circadian clock gating of ABA signaling is crucial for plant drought adaptation.
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