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Finding a Needle in a Haystack: Proteomics in Heart Failure.
Sam A Michelhaugh1, James L Januzzi1,2,3
1Department of Medicine, Division of Cardiology, Massachusetts General Hospital, Boston, Massachusetts.
JACC. Basic to Translational Science
|November 4, 2020
Summary
Proteomics advances enable the discovery of circulating protein biomarkers for heart failure (HF). These biomarkers improve understanding of HF pathways and identify potential therapeutic targets.
Area of Science:
- Biochemistry
- Cardiology
- Proteomics
Background:
- Circulating protein biomarkers offer insights into heart failure (HF) pathophysiology.
- Proteomics advancements allow for sensitive and selective measurement of numerous proteins.
Purpose of the Study:
- To provide an overview of proteomic methods used in heart failure research.
- To highlight the contribution of different proteomic techniques to understanding HF pathways.
Main Methods:
- Review of various proteomic techniques including mass spectrometry, protein microarrays, aptamers, and proximity extension assays.
- Discussion of the advantages of each proteomic method.
Main Results:
- Proteomics enables the identification of previously unrecognized pathways in HF.
- Low abundance proteins can be detected, aiding biomarker discovery.
- Potential therapeutic targets for HF can be identified through proteomic analysis.
Conclusions:
- Proteomic methods are valuable tools for advancing heart failure research.
- Understanding HF pathways through proteomics can lead to novel therapeutic strategies.
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