Controlled Nitric Oxide Release Using Poly(lactic-co-glycolic acid) Nanoparticles for Anti-Inflammatory Effects

Area of Science:

• Biomaterials Science
• Nanotechnology
• Immunology

Background:

• Nitric oxide (NO) is crucial for immune regulation and inflammation but has limitations for therapeutic use due to its short half-life.
• Controlled delivery systems are essential to overcome NO's limitations and achieve therapeutic efficacy in biomedical applications.
• Existing NO delivery methods often suffer from burst release, hindering sustained therapeutic effects.

Purpose of the Study:

• To fabricate a poly(lactic-co-glycolic acid) (PLGA)-based nanoparticle system for sustained nitric oxide (NO) release.
• To investigate the controlled release profile of NO from the developed system under physiological conditions.
• To evaluate the efficacy of the NO delivery system in regulating inflammatory responses.

Main Methods:

• Fabrication of PLGA nanoparticles encapsulating branched polyethylenimine diazeniumdiolate (BPEI/NONOate), a pH-responsive NO donor.
• Characterization of nanoparticle properties and assessment of NO release kinetics under physiological conditions.
• In vitro evaluation of the system's ability to modulate inflammatory responses in lipopolysaccharide-stimulated peripheral blood mononuclear cells (PBMCs).

Main Results:

• The PLGA nanoparticles demonstrated sustained release of NO, preventing initial burst release.
• The stabilization effect from BPEI/NONOate amine group interactions ensured a consistent low-level NO release.
• The controlled NO release effectively regulated inflammatory responses in stimulated PBMCs.

Conclusions:

• A novel PLGA-based nanoparticle system effectively achieves sustained and controlled nitric oxide delivery.
• This system demonstrates significant potential for modulating inflammatory responses in a therapeutic context.
• The developed NO delivery carrier offers a promising strategy for biomedical applications requiring precise NO modulation.