Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.0K
Atomic Force Microscopy01:08

Atomic Force Microscopy

4.0K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.0K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.5K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.5K
X-ray Imaging01:24

X-ray Imaging

9.4K
German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
9.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gepta-EX: a multi-channel germanium detector for X-ray absorption fine structure.

Journal of synchrotron radiation·2026
Same author

Recommendations and considerations for hydroxyl radical protein footprinting-mass spectrometry.

Nature methods·2026
Same author

A bifunctional H/ACA snoRNP mediates both pseudouridylation and rRNA scaffolding during ribosome assembly.

bioRxiv : the preprint server for biology·2026
Same author

High-resolution in-beam video rate imaging with the ClearXCam for synchrotron beamlines.

Journal of synchrotron radiation·2026
Same author

Hydroxyl radical footprinting modification reveals an intradomain communication pathway in EFL1 disrupted by a Shwachman-Diamond syndrome-associated mutation.

Protein science : a publication of the Protein Society·2026
Same author

Evaluation of an event-driven 3FI ASIC for spectroscopic X-ray detection with synchrotron radiation.

Journal of synchrotron radiation·2025
Same journal

Launching a new era for Short Communications in Journal of Synchrotron Radiation.

Journal of synchrotron radiation·2026
Same journal

Sagittal collimating diaboloid: a new grazing-incidence mirror surface for higher-throughput resonant inelastic X-ray scattering spectrometers.

Journal of synchrotron radiation·2026
Same journal

Synchrotron X-ray tomography and spectroscopy in numismatics: disclosing counterfeit practices in medieval silver coins.

Journal of synchrotron radiation·2026
Same journal

The Big Data Science Center at the Shanghai Synchrotron Radiation Facility: the architecture of the superfacility.

Journal of synchrotron radiation·2026
Same journal

A robotic and high-throughput X-ray micro-computed tomography workflow.

Journal of synchrotron radiation·2026
Same journal

Evolution of hierarchical phase-contrast tomography on the European Synchrotron beamlines BM05 and BM18: a whole adult human brain imaging case study.

Journal of synchrotron radiation·2026
See all related articles

Related Experiment Video

Updated: Dec 2, 2025

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
10:12

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

Published on: June 19, 2018

9.4K

A coded aperture microscope for X-ray fluorescence full-field imaging.

D P Siddons1, A J Kuczewski1, A K Rumaiz1

  • 1National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, NY 11973, USA.

Journal of Synchrotron Radiation
|November 4, 2020
PubMed
Summary
This summary is machine-generated.

This study introduces a novel X-ray microscope using coded apertures for full-field imaging. This design achieves micrometer resolution without sample scanning, enhancing X-ray microscopy capabilities.

Keywords:
X-ray microscopecoded aperturefluorescencefull-field

More Related Videos

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
07:54

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

Published on: March 12, 2015

9.7K
Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging
12:27

Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging

Published on: November 25, 2009

9.8K

Related Experiment Videos

Last Updated: Dec 2, 2025

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
10:12

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

Published on: June 19, 2018

9.4K
Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
07:54

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

Published on: March 12, 2015

9.7K
Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging
12:27

Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging

Published on: November 25, 2009

9.8K

Area of Science:

  • X-ray microscopy
  • Optics
  • Materials Science

Background:

  • Traditional X-ray microscopes often require sample scanning, limiting imaging speed and applicability.
  • Achieving high spatial resolution in X-ray microscopy necessitates achromatic optics that are energy-independent.
  • Simple pinhole optics have low throughput, hindering practical applications.

Purpose of the Study:

  • To design and construct an instrument for full-field X-ray fluorescence imaging.
  • To develop an X-ray microscope with micrometer spatial resolution that eliminates the need for sample scanning.
  • To utilize coded apertures for improved throughput and achromatic imaging in X-ray microscopy.

Main Methods:

  • Fabrication of modified uniformly redundant arrays (MURAs) using gold lithography on silicon nitride membranes.
  • MURAs feature 10 µm openings, 50% open area, and gold thickness of 25 µm for contrast up to 20 keV.
  • Silicon nitride membranes offer transparency in the soft X-ray region; various MURA orders and their negatives were produced.

Main Results:

  • Successful fabrication of MURAs with specified dimensions and material properties.
  • The developed MURA-based system enables full-field imaging, bypassing the need for sample scanning.
  • The use of both positive and negative masks aids in reducing artifacts and correcting contrast.

Conclusions:

  • The presented instrument demonstrates a viable approach to high-resolution, full-field X-ray fluorescence microscopy.
  • Coded apertures, specifically MURAs, offer an effective solution for achromatic and high-throughput X-ray imaging.
  • This technology has the potential to advance various fields requiring detailed elemental analysis of samples.