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Updated: Dec 1, 2025

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Published on: May 3, 2013

The fluorescence toolbox for visualizing autophagy.

Siyang Ding¹, Yuning Hong

¹Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne Victoria 3086, Australia. Y.Hong@latrobe.edu.au.

Chemical Society Reviews

|November 6, 2020

Summary

This summary is machine-generated.

Autophagy, a cell survival process, involves digesting damaged components. This review details fluorescence techniques for monitoring autophagy dynamics, crucial for understanding disease and developing therapies.

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Last Updated: Dec 1, 2025

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Published on: May 3, 2013

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae

Published on: July 18, 2011

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Published on: July 21, 2017

Area of Science:

Cell Biology
Biochemistry
Molecular Biology

Background:

Autophagy is a fundamental cellular process essential for survival under stress and for clearing cellular debris.
Dysregulation of autophagy is implicated in various human diseases, underscoring its therapeutic potential.
Accurate methods for monitoring autophagy are vital but currently lack comprehensive establishment.

Purpose of the Study:

To review current fluorescence-based techniques for visualizing and measuring intracellular autophagy.
To categorize these methods based on detected targets and fluorescence tool types.
To discuss their applications in biomedical science and future prospects.

Main Methods:

Overview of major fluorescence-based techniques for autophagy detection.
Categorization by target molecules (e.g., autophagosomes, lysosomes) and fluorescence modalities (e.g., microscopy, flow cytometry).
Analysis of working mechanisms and utility in cellular and tissue contexts.

Main Results:

Detailed description of various fluorescence tools and their principles.
Highlighting the strengths and limitations of different techniques.
Illustrating the application of these methods in studying autophagy-related biological processes.

Conclusions:

Fluorescence-based methods are indispensable for studying autophagy dynamics.
Further development is needed to enhance sensitivity, specificity, and quantitative accuracy.
These techniques hold significant promise for advancing autophagy research and therapeutic strategies.