Small molecule-PNA oligomer conjugates for rRNA A-site at neutral pH for FID assays
En Ting Tabitha Lee1, Yusuke Sato, Seiichi Nishizawa
1Department of Chemistry, Graduate School of Science, Tohoku University, Sendai 980-8578, Japan. yusuke.sato.a7@tohoku.ac.jp seiichi.nishizawa.c8@tohoku.ac.jp.
Abstract:
A triplex-forming PNA oligomer conjugated with a naphthyridine derivative (ATMND-C2-NH2) showed high selectivity and strong binding for the bacterial rRNA A-site at pH 7.0 (Kd = 190 ± 72 nM), which was accompanied by fluorogenic signaling that allowed the potential use of this conjugate probe in fluorescent indicator displacement assays.
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